CovidV2, Main, Exploration, bibRecord, 001C22

Monolayer culture of rat parotid acinar cells without basement membrane substrates

Identifieur interne : 001C22 ( Main/Exploration ); précédent : 001C21; suivant : 001C23

Monolayer culture of rat parotid acinar cells without basement membrane substrates

Auteurs : Cheryl S. Kiser [États-Unis] ; Firoz Rahemtulla [États-Unis] ; Britta M Nsson-Rahemtulla [États-Unis]

Source :

In Vitro Cellular & Developmental Biology [ 0883-8364 ] ; 1990-09-01.

RBID : ISTEX:5F565427808DE6AB4854FDC68C705FFD854DE58B

English descriptors

Abstract

Summary: Acinar cells have been difficult to maintain in primary or secondary cultures over extended periods of time. The most successful monolayer culture system reported to date requires basement membrane substrates. We report here a technique for culture of rat parotid acinar cells which does not rely upon basement membrane supports for maintenance and growth. The procedure involves gland excision, treatment to chelate metal ions, enzymatic digestion with collagenases and hyaluronidase, removal of fat and red blood cells by gravimetric separation, and nylon mesh filtration to yield a homogeneous suspension of small aggregates and single cells. The cells were examined for: a) morphology, identity, and growth; b) macromolecular synthesis; and c) secretory output. They were healthy, peroxidase positive, and growing for up to 10 d. Protein synthesis increased from the point of cell layer formation at 3 to 4 d, through 10 d, while DNA synthesis decreased. As in other studies, amylase secretion fell sharply between 2 and 4 d in culture and remained low. Although previous studies indicated that the initial isolation protocol left these acinar cells unable to thrive in monolayer culture except in the presence of basement membrane substrates, the modified technique reported herein allows these cells to attach, spread, and grow on a wide variety of commerically available plasticware. this method lends itself readily to long-term analysis of rat parotid acinar cell metabolism without the complications of dedifferentiation, cell loss through culture manipulation common in suspension cultures, or complex interactions between bioactive supports and cell surfaces.

Url:

https://api.istex.fr/ark:/67375/1BB-N7BHSVRG-F/fulltext.pdf

DOI: 10.1007/BF02624613

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000727
to stream Istex, to step Curation: 000694
to stream Istex, to step Checkpoint: 000607
to stream Main, to step Merge: 001C42
to stream Main, to step Curation: 001C22

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Monolayer culture of rat parotid acinar cells without basement membrane substrates</title>
<author><name sortKey="Kiser, Cheryl S" sort="Kiser, Cheryl S" uniqKey="Kiser C" first="Cheryl S." last="Kiser">Cheryl S. Kiser</name>
</author>
<author><name sortKey="Rahemtulla, Firoz" sort="Rahemtulla, Firoz" uniqKey="Rahemtulla F" first="Firoz" last="Rahemtulla">Firoz Rahemtulla</name>
</author>
<author><name sortKey="M Nsson Rahemtulla, Britta" sort="M Nsson Rahemtulla, Britta" uniqKey="M Nsson Rahemtulla B" first="Britta" last="M Nsson-Rahemtulla">Britta M Nsson-Rahemtulla</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:5F565427808DE6AB4854FDC68C705FFD854DE58B</idno>
<date when="1990" year="1990">1990</date>
<idno type="doi">10.1007/BF02624613</idno>
<idno type="url">https://api.istex.fr/ark:/67375/1BB-N7BHSVRG-F/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000727</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000727</idno>
<idno type="wicri:Area/Istex/Curation">000694</idno>
<idno type="wicri:Area/Istex/Checkpoint">000607</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000607</idno>
<idno type="wicri:doubleKey">0883-8364:1990:Kiser C:monolayer:culture:of</idno>
<idno type="wicri:Area/Main/Merge">001C42</idno>
<idno type="wicri:Area/Main/Curation">001C22</idno>
<idno type="wicri:Area/Main/Exploration">001C22</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Monolayer culture of rat parotid acinar cells without basement membrane substrates</title>
<author><name sortKey="Kiser, Cheryl S" sort="Kiser, Cheryl S" uniqKey="Kiser C" first="Cheryl S." last="Kiser">Cheryl S. Kiser</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Alabama</region>
</placeName>
<wicri:cityArea>Department of Community and Public Health Dentistry, University of Alabama School of Dentistry, 35294, Birmingham</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Rahemtulla, Firoz" sort="Rahemtulla, Firoz" uniqKey="Rahemtulla F" first="Firoz" last="Rahemtulla">Firoz Rahemtulla</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Alabama</region>
</placeName>
<wicri:cityArea>Department of Oral Biology, University of Alabama School of Dentistry, 35294, Birmingham</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="M Nsson Rahemtulla, Britta" sort="M Nsson Rahemtulla, Britta" uniqKey="M Nsson Rahemtulla B" first="Britta" last="M Nsson-Rahemtulla">Britta M Nsson-Rahemtulla</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Alabama</region>
</placeName>
<wicri:cityArea>Department of Community and Public Health Dentistry, University of Alabama School of Dentistry, 35294, Birmingham</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">In Vitro Cellular & Developmental Biology</title>
<title level="j" type="sub">Journal of the Tissue Culture Association</title>
<title level="j" type="abbrev">In Vitro Cell Dev Biol</title>
<idno type="ISSN">0883-8364</idno>
<idno type="eISSN">1475-2689</idno>
<imprint><publisher>Springer-Verlag</publisher>
<pubPlace>New York</pubPlace>
<date type="published" when="1990-09-01">1990-09-01</date>
<biblScope unit="volume">26</biblScope>
<biblScope unit="issue">9</biblScope>
<biblScope unit="page" from="878">878</biblScope>
<biblScope unit="page" to="888">888</biblScope>
</imprint>
<idno type="ISSN">0883-8364</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0883-8364</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>acinar cell culture</term>
<term>basement membrane</term>
<term>parotid gland</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Absorbance measurements</term>
<term>Absorbance values</term>
<term>Acinar</term>
<term>Acinar cell culture</term>
<term>Acinar cell cultures</term>
<term>Acinar cells</term>
<term>Acute secretagogue stimulation</term>
<term>Aggregate attachment</term>
<term>Amylase</term>
<term>Amylase activity</term>
<term>Amylase levels</term>
<term>Amylase secretion</term>
<term>Amylase test</term>
<term>Assay</term>
<term>Atmosphere incubator</term>
<term>Average spread</term>
<term>Basement membrane</term>
<term>Basement membrane substrates</term>
<term>Becton dickinson</term>
<term>Biol</term>
<term>Cary model</term>
<term>Cell biol</term>
<term>Cell dispersion</term>
<term>Cell layer formation</term>
<term>Cell layers</term>
<term>Cell loss</term>
<term>Cell suspension</term>
<term>Cell viability</term>
<term>Collaborative research</term>
<term>Collagenase type</term>
<term>Complete medium</term>
<term>Constance oliver</term>
<term>Culture conditions</term>
<term>Culture medium</term>
<term>Culture period</term>
<term>Eastman kodak</term>
<term>Electron microscopy</term>
<term>Enzyme activities</term>
<term>Epidermal growth factor</term>
<term>Epinephrine</term>
<term>Epithelial cells</term>
<term>Equal volumes</term>
<term>Falcon</term>
<term>Falcon primaria</term>
<term>Falcon primaria dishes</term>
<term>Falcon primaria plasticware</term>
<term>Filtration</term>
<term>Human gingival fibroblasts</term>
<term>Human serum</term>
<term>Hyaluronidase</term>
<term>Incubation buffer</term>
<term>Incubation period</term>
<term>Isoproterenol</term>
<term>Large aggregates</term>
<term>Linbro</term>
<term>Linbro plasticware</term>
<term>Liquid scintillation counter</term>
<term>Loose cells</term>
<term>Macromolecular synthesis</term>
<term>Matrigel</term>
<term>Maximal attachment</term>
<term>Membrane</term>
<term>Mesh filtration</term>
<term>Model centrifuge</term>
<term>Molecular weight</term>
<term>Monolayer culture</term>
<term>Nippon kogaku</term>
<term>Nitex nylon mesh screen</term>
<term>Nylon mesh filtration</term>
<term>Oral biol</term>
<term>Organ culture</term>
<term>Palo alto</term>
<term>Pancreatic acinar cells</term>
<term>Parotid</term>
<term>Parotid acinar cells</term>
<term>Parotid cells</term>
<term>Parotid gland</term>
<term>Parotid glands</term>
<term>Pellet</term>
<term>Peroxidase</term>
<term>Peroxidase activity</term>
<term>Peroxidase staining</term>
<term>Peroxidase system</term>
<term>Personal communication</term>
<term>Phadebas amylase test</term>
<term>Phadebas humylase control</term>
<term>Pharmacia diagnostics</term>
<term>Plastek</term>
<term>Previous studies</term>
<term>Primaria</term>
<term>Primary cultures</term>
<term>Protein synthesis</term>
<term>Reagent blanks</term>
<term>Reconstituted basement membrane</term>
<term>Rinsed</term>
<term>Room temperature</term>
<term>Rotary stirrer</term>
<term>Salivary</term>
<term>Salivary glands</term>
<term>Salivary peroxidase</term>
<term>Salivary peroxidase activity</term>
<term>Same buffer</term>
<term>Same pattern</term>
<term>Scanning densitometry</term>
<term>Secondary cultures</term>
<term>Secretagogue</term>
<term>Secretion</term>
<term>Secretory</term>
<term>Secretory granules</term>
<term>Secretory output</term>
<term>Sigma</term>
<term>Single cells</term>
<term>Special substrate</term>
<term>Standard curve</term>
<term>Submandibular duct complexes</term>
<term>Tetmak corp</term>
<term>Thymidine incorporation</term>
<term>Tissue dissociation</term>
<term>Tumor extracts</term>
<term>Varian instrument division</term>
<term>Viability</term>
<term>Viable cells</term>
<term>Wide variety</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Summary: Acinar cells have been difficult to maintain in primary or secondary cultures over extended periods of time. The most successful monolayer culture system reported to date requires basement membrane substrates. We report here a technique for culture of rat parotid acinar cells which does not rely upon basement membrane supports for maintenance and growth. The procedure involves gland excision, treatment to chelate metal ions, enzymatic digestion with collagenases and hyaluronidase, removal of fat and red blood cells by gravimetric separation, and nylon mesh filtration to yield a homogeneous suspension of small aggregates and single cells. The cells were examined for: a) morphology, identity, and growth; b) macromolecular synthesis; and c) secretory output. They were healthy, peroxidase positive, and growing for up to 10 d. Protein synthesis increased from the point of cell layer formation at 3 to 4 d, through 10 d, while DNA synthesis decreased. As in other studies, amylase secretion fell sharply between 2 and 4 d in culture and remained low. Although previous studies indicated that the initial isolation protocol left these acinar cells unable to thrive in monolayer culture except in the presence of basement membrane substrates, the modified technique reported herein allows these cells to attach, spread, and grow on a wide variety of commerically available plasticware. this method lends itself readily to long-term analysis of rat parotid acinar cell metabolism without the complications of dedifferentiation, cell loss through culture manipulation common in suspension cultures, or complex interactions between bioactive supports and cell surfaces.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Alabama</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Alabama"><name sortKey="Kiser, Cheryl S" sort="Kiser, Cheryl S" uniqKey="Kiser C" first="Cheryl S." last="Kiser">Cheryl S. Kiser</name>
</region>
<name sortKey="M Nsson Rahemtulla, Britta" sort="M Nsson Rahemtulla, Britta" uniqKey="M Nsson Rahemtulla B" first="Britta" last="M Nsson-Rahemtulla">Britta M Nsson-Rahemtulla</name>
<name sortKey="Rahemtulla, Firoz" sort="Rahemtulla, Firoz" uniqKey="Rahemtulla F" first="Firoz" last="Rahemtulla">Firoz Rahemtulla</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C22 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001C22 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    CovidV2
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:5F565427808DE6AB4854FDC68C705FFD854DE58B
   |texte=   Monolayer culture of rat parotid acinar cells without basement membrane substrates
}}

This area was generated with Dilib version V0.6.33.
Data generation: Sat Mar 28 17:51:24 2020. Site generation: Sun Jan 31 15:35:48 2021

	Serveur d'exploration Covid (26 mars)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration Covid (26 mars)

Monolayer culture of rat parotid acinar cells without basement membrane substrates

Monolayer culture of rat parotid acinar cells without basement membrane substrates

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri